Fusion with heat-resistant obscure (Hero) proteins have the potential to improve the molecular property of recombinant proteins

Heroタンパク質が、目的タンパク質の可溶化・安定化融合タグとして応用可能なことを明らかにした。(現在、本論文およびPLOS Biol. 2020の知見と合わせ、GTIE GAPファンドによってスタートアップを目指した実証実験を行っている。)

Although recombinant proteins are widely used in biotechnology and pharmaceutical industries, improving their solubility and stability is often a challenging issue. We recently discovered a class of highly unstructured heat-resistant obscure (Hero) proteins, which function to protect other “client” proteins in trans from various stresses in vitro and in vivo. Here, we show that fusion of Hero proteins in cis can enhance the molecular property of recombinant proteins. Fusion with Hero11 improved the otherwise challenging production of TAR DNA-binding protein of 43 kDa (TDP-43) in Escherichia coli. Moreover, fusing with Hero9 strongly protected the activity of firefly luciferase bearing destabilizing mutations against heat and other stress conditions. These data suggest that Hero proteins have the potential to be used as versatile stabilization tags for recombinant protein production.

Authors: Morimoto E, Tsuboyama K, Tomari Y

Journal: PLoS One.2022 ;17(6):e0270097.