We introduced an isotopically labeled RNA aptamer for HIV-1 Tat prepared by E. coli transcription into HeLa cells. We successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study the interaction of the RNA aptamer with argininamide in living human cells with higher resolution.
Authors: Eladl, O., Yamaoki, Y., Kondo, K., Nagata, T. and Katahira, M.
Journal: Chem. Commun. 2022, 59, 102
- 研究成果2023.10.20NMR characterization of the structure of the intrinsically disordered region of human origin recognition complex subunit 1, hORC1, and of its interaction with G-quadruplex DNAs
- 研究成果2023.09.29Shedding light on the base-pair opening dynamics of nucleic acids in living human cells
- 研究成果2023.09.29Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR
- 研究成果2023.09.29Complex Formation of an RNA Aptamer with a Part of HIV-1 Tat through Induction of Base Triples in Living Human Cells Proven by In-Cell NMR